CHANGES IN HEAT SHOCK PROTEIN SYNTHESIS AND THERMOTOLERANCE OF ARABIDOPSIS THALIANA SEEDLINGS AS A RESULT OF INHIBITION
OF Hsp90 BY GELDANAMYCIN
L.Ye. Kozeko
Institute of Botany, National Academy of Sciences of Ukraine, Kiev;
e-mail: liudmyla.kozeko@gmail.com
The influence of geldanamycin (GA), which is a specific inhibitor of heat shock protein Hsp90 activities, on synthesis of Hsp70 and Hsp90 and thermotolerance of Arabidopsis thaliana
seedlings has been studied. Incubation of seedlings with GA was shown to induce synthesis of these stress proteins under normal conditions. Treatment of seeds with the Hsp90 inhibitor
resulted in the elevated constitutive levels of Hsp70 and Hsp90 in seedlings as well as increased induction of their synthesis under heat shock, at that the effect of GA increased with its
concentration. These up-regulation of Hsp promoted thermotolerance of seedlings. The obtained results are considered as evidence for autoregulation of heat shock protein synthesis and
regulation of plant tolerance by Hsp90.
Key words: heat shock proteins, geldanamycin, stress reaction, thermotolerance, Arabidopsis thaliana
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