THE EFFECT OF CYCLOOXYGENASE AND LIPOXYGENASE INHIBITORS ON Ca2+-RESPONSES INDUCED BY GLUTOXIM AND
MOLIXAN IN MACROPHAGES
L.S. Kurilova, Z.I. Krutetskaya, A.A. Naumova, S.N. Butov, N.I. Krutetskaya, V.G. Antonov
St. Petersburg State University;
e-mail: cozzy@mail.ru
Glutoxim and molixan belong to a new generation of disulfide-containing drugs with immunomodulatory, hepatoprotective and hemopoetic effect. Using Fura-2AM microfluorimetry,
the possible involvement of the cyclooxygenase and lipoxygenase pathways of arachidonic acid oxidation in the effect of glutoxim and molixan on the intracellular Ca2+
concentration in rat peritoneal macrophages has been investigated. We have shown for the first time that preincubation of the cells with the cyclooxygenase inhibitors, indomethacin
and aspirin, or li-poxygenase inhibitors, nordihydroguaiaretic acid, caffeic acid and baicalein, almost completely prevents the intracellular Ca2+ concentration increase
induced by glutoxim or molixan. The obtained data indicate the involvement of products and/or enzymes of the arachidonic acid cyclooxygenase and lipoxygenase metabolism
pathways in the effect of glutoxim and molixan on the processes of Ca2+ signaling in macrophages.
Key words: intracellular Ca2+ concentration, peritoneal macrophages, glutoxim, molixan,
arachidonic acid, cyclooxygenases, lipoxygenases
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