CHARACTERISTICS OF MIGRATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STROMAL CELLS AFTER CO-CULTIVATION WITH
ACTIVATED MONOCYTES IN VITRO
O.A. Grigoryeva,1,* I.V. Korovina,1 B.Sh. Gogia,2 V.Yu. Sysoeva 1
1 M. V. Lomonosov Moscow State University and 2 Institute of Surgery named after A. V. Vishnevsky, Moscow;
* e-mail: go.grigorievaolga@gmail.com
Mesenchymal stromal cells (MSC) are considered to be promising tool of regenerative medicine. Migration of MSC toward damaged inflammatory site is essential for
physiological tissue reparation. Therefore we studied modifications of migratory features of adipose tissue derived MSC (AT-MSC) after co-cultivation with activated monocytes
derived from THP-1 cell line. As a result, we have observed an increased migration rate of AT-MSC in vitro in the absence of chemoattractant gradient as well as toward the
gradient of PDGF BB (platelet-derived growth factor BB), which is well known chemoattractant for the cells of mesenchymal origin. Furthermore, the rate of directional AT-MSC
migration through fibronectin was also increased. We have established that signaling from PDGFRb which is activated through binding of integrin receptors with extracellular
matrix may be possible way to stimulate cellular migration under simulated inflammatory conditions.
Key words: mesenchymal stromal cells, cell migration, inflammation, PDGFR-β, integrines
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