EFFECT OF BONE MARROW CELLS TRANSPLANTATION ON THE DECIDUA FORMATION
IN PSEUDOPREGNANT RATS
A.P. Domnina,1,* V.M. Mikhailov,1 N.N. Nikolsky 1,2
1 Institute of Cytology RAS, St. Petersburg, and 2 St. Petersburg State Polytechnical University;
* e-mail: aldomnina@mail.ru
One of the most common causes of the current pregnancy loss is the failure of the decidual reaction of endometrial cells. It is assumed that a partial source of decidual cells in
endometrial tissue is bone marrow cells (BMCs). In the present work, we have studied possible effect of BMCs transplantation on the process of decidualization using the model
of pseudopregnancy in rats. BMCs were flushed from the rat femurs and tibias. The obtained suspension of single BMCs was injected into one of rat uterine horns on the 5th day
of pseudopregnancy. PBS without cells was injected into the contralateral horn served as the control. Rats were sacrificed on the 11th day of pseudopregnancy. Decidua formed
in the experimental uterine horn showed an increase in the mesoantimezometral direction of their diameter of about 1.5-2 times as compared with a control horn. The weight of
decidual tissue in the experimental horn exceeded 3 times the weight of the control one. The presence of transplanted BMCs in decidual tissue was documented by preliminary
double staining of BMCs with membrane dye PKH 26 Red and nuclear dye Hoechst 33342. Histological analysis of decidua sections after transplantation revealed any alterations
neither in cell differentiation nor in tissue structure. We conclude that BMCs transplantation stimulates decidualization in animals.
Key words: decidual tissue, decidual cells, bone marrow stem cells, pseudopregnancy
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