EFFECTS OF DOPAMINE AND ADENOSINE ON REGULATION OF WATER-ELECTROLYTE
EXCHANGE IN AMOEBA PROTEUS
Ya.Yu. Bagrov,1 N.B. Manusova
I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, St. Petersburg;
1 e-mail: a.polyanichko@spbu.ru
Dopamine and adenosine both regulate transport of sodium chloride in the renal tubules in mammals. We have studied the effect of dopamine and adenosine on spontaneous
activity of contractile vacuole of Amoeba proteous. Both substances stimulated contractile vacuole. The effect of dopamine was suppressed by D2 receptor antagonist,
haloperidol, but not by D1 antagonist, SCH 39166. Adenylate cyclase inhibitor, 2.5-dideoxyade-nosine, suppressed the effect of dopamine, but not of adenosine. Inhibitor
of protein kinase C, staurosporine, in contrast, blocked the effect of adenosine, but not dopamine. Notably, dopamine opposed effect of adenosine and vice versa. These
results suggest that similar effects of dopamine and adenosine could be mediated by different intracellulare mechanisms.
Key words: protozoa, Amoeba proteus, contractile vacuole, water-salt homeostasis, adenosine, dopamine
| Back
| Contents
| Main |
