BDNF SECRETION IN HUMAN MESENCHYMAL STEM CELLS ISOLATED FROM BONE MARROW, ENDOMETRIUM AND ADIPOSE TISSUE
V.I. Zemelko,1 I.V. Kozhucharova,1,2 Z.V. Kovaleva,1,2 A.P. Domnina,1
N.A. Pugovkina,1,2 I.I. Fridlyanskaya,1,2 M.V. Puzanov,1 S.V. Anisimov,1,2 T.M. Grinchuk,1,2
N.N. Nikolsky 1,3
1 Institute of Cytology RAS, 2 V. A. Almazov Federal Center for Heart, Blood and Endocrinology, St. Petersburg,
and 3 St. Petersburg State Polytechnic University;
e-mail: vzemelko@mail.ru
The ability of mesenchymal stem cells (MSCs) to differentiate into neuronal lineage determines the potential of these cells as a substrate for a cell replacement therapy. In this paper we
compare the neurogenic potential of MSCs isolated from bone marrow (BMSC), subcutaneous adipose tissue (AD MSC) and menstrual blood (eMSC). It was found that the native eMCSs,
BMSCs and AD MSCs express neuronal marker b-III-tubulin with a frequency of 90, 50 and 14 %, respectively. We also showned that eMSCs have a high endogenous level of brain-derived
neurotrophic factor (BDNF), whereas the BMSCs and the AD MSCs are characterized by low basal BDNF levels. As induction of neuronal differentiation in the studied MSCs using differentiation
medium containing B27 and N2 supplements, 5-azacytidine, retinoic acid, IBMX and dbcAMF caused changes in the cells morphology, the increased expression of b-III-tubulin, and the
appearance of neuronal markers GFAP, NF-H, NeuN and MAP2. BDNF secretion during differentiation was significantly enhanced in the BMSCs and decreased in the eMSCs cultures.
However, no correlation between the basal and induced levels of the neuronal markers expression and BDNF secretion in the studied MSCs has been established.
Key words: cell therapy, mesenchymal stem cells, neurotrophic factors
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