THE USE OF APOPTOSIS INDUCERS IN THE THERAPY OF EXPERIMENTAL INFLUENZA
INFECTION AND PREVENTING OF CHRONIC POST-INFLUENZA LUNG DAMAGE
V.V. Zarubaev,1 V.G. Tribulovich,1 S.V. Belyaevskaya,1 N.A. Barlev,2 O.I. Kiselev 1
1 Influenza Research Institute, Ministry of Health Care of Russian Federation,
and 2 Institute of Cytology RAS, St. Petersburg;
e-mail: zarubaev@influenza.spb.ru
Influenza is a respiratory infection widely spread around the world. Influenza complications are various in
nature and in most cases involve the excessive proliferation of cells in respiratory tract as a factor of pathogenesis.
In the present work the efficacy of the use of apoptosis inducer 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphtalenecarboxylic
acid (AHPN) for prophylaxis of chronic damage on the stage of post- influenza pneumonia
has been studied. Mice were infected with influenza virus A/mallard/Pennsylvania/10218/84(H5N2) with further
study of the level of influenza virus reproduction in the lungs, specific mortality of animals and morphology
of the foci of post-influenza pneumonia on the 15th day post inoculation. AHPN was shown to decrease the
infectious activity of the virus in the lungs by 1.2—1.5 log10 EID50/0.2 mL depending on the dose as compared
to the control group, in a weak decrease in mortality of animals (protection index was 12.5—37.5 %). The application
of AHPN restricted both the proliferative and infiltrative component in chronic post-influenza lesions.
It demonstrated the most pronounced effect on the lung morphology when applied on days 4 to 7 post inoculation,
i. e. in the period of maximal activation of inflammatory tissue infiltration and regeneration of bronchiolar
epithelium. In conclusion, the use of apoptosis inducers can partially prevent the development of chronic
post-influenza lesions with proliferative component.
Key words: influenza, post-influenza pneumonia, apoptosis, apoptosis inducers, AHPN, chemotherapy
| Back
| Contents
| Main |
|
