COLOCALIZATION OF NUCLEOLI IN CELL NUCLEI OF HeLa LINE
Yu.P. Petrov,1,* Yu.A. Negulyaev,1,2 N.V. Tsupkina 1
1 Institute of Cytology RAS, St. Petersburg; and 2 Chair of Physical and Chemical Biology of the Cell the St. Petersburg State Polytechnical University;
* e-mail: yupe3ov@mail.ru
The pattern of localization of nucleoli relative to each other and to cell nucleus was studied in M-HeLa cell line. For this puspose, the following morphometric parameters were introduced.
For the two-nucleolar cells: 1) the ratio of the nucleus long axis to the length of a segment between the centers of the nucleoli, and 2) the angle between the segment connecting the centers
of the nucleoli and a longitudinal axis of cell nucleus. For the three-nucleolar cells: the ratio perimeter of the nucleus to perimeter of a triangle with vertexes in the centre of nucleoli. We have
shown that the values of these parameters are individual for each cell but their values remain constant for the cell in spite of the changes in cell shape. These results allow us to conclude that,
on the one hand, the nucleoli colocalization is individual for each cell, and, on the other hand, location of nucleoli in relation to nucleus is not changed during interphase. Thereby, the distance
between nucleoli increases proportionally with nucleus growth.
Key words: nucleolus, nucleus axis, interphase, cell morphometry
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