Tsitologiya  2014  56 (3) : 218–224

DESIGN OF CELL LINE STABLE EXPRESSING PROTEASOMAL SUBUNIT PSMD14 FUSED TO THE FLUORESCENT PROTEIN EGFP AND HTBH TAG BASED ON HEK293 CELLS V.A. Kulichkova,1 Yu.Ya. Zaykova,1 Yu.B. Ermolaeva,1 E.V. Vinogradova,2 L. Huang,3 A.N. Tomilin,1 N.A. Barlev,1,4,5 A.S. Tsimokha 1,* 1 Institute of Cytology RAS, St. Petersburg, 2 St. Petersburg State University, 3 University of California, USA, 4 St. Petersburg State Institute of Technology and 5 University of Leicester, UK; * e-mail: atsimokha@mail.cytspb.rssi.ru A stable cell line based on HEK293 cells that expresses proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag has been selected. This chimera was shown to be incorporated completely into proteolitic active proteasomes. The created cell line can be used for further fluorescent studies of proteasomes localization in the cell. Key words:  affinity purification, chymotrypsin-like peptidase activity, green fluorescent protein, proteasome |  Back   |  Contents   |  Main  |