RESPONSE OF HeLa CELLS TO MITOMYCINE C. III. THE ANALYSIS OF NUCLEOLI
OF MOTHER AND DAUGHTER CELLS
Yu.P. Petrov,1,* Yu.A. Negulyaev,1,2 N.V. Tsupkina 1
1 Institute of Cytology RAS, St. Petersburg, and 1 Chair of Physical and Chemical Biology of the Cell,
St. Petersburg State Polytechnical University;
*e-mail: yupe3ov@mail.ru
The comparative analysis of the number of nucleoli in cells of the established HeLa-M line was carried out
before and after exposure to mitomycin C in a concentration of 10 mg/ml for 2 h. Using time-lapse microscopy,
nucleoli in mother and their respective daughter cells were computed. It has been shown that the average number
of nucleoli per cell is generally higher in daughter cells than in mother cells, and a standard deviation, on
the contrary, decreases. An average number of nucleoli in daughter cells, whose mother cells had been treated
with mitomycin C, was higher than in corresponding cells of control group. The separate analysis has been performed
for the cells having from 1 to 4 nucleoli. Nonrandom complete coincidence of the number of nucleoli in
mather and daughter cells has been typicaly shown for about 1/7 of the total cell population. Mitomycin C reduces
this value of about 1.5 times.
Key words: a nucleus, a nucleolus, a daughter cell, a mother cell
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