FEATURES OF CYTOGENETIC CHANGES IN THE HUMAN LYMPHOMA U-937 CELLS
AFTER INDUCTION OF APOPTOSIS BY TUMOR NECROSIS FACTOR
O.A. Kovaleva, Ya.S. Yasinskiy, N.A. Bezdeneznykh, Yu.I. Kudryavets
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kiev;
e-mail: strukov2002@mail.ru
A comparative analysis of the frequency of cytogenetic abnormalities in cell population U-937 in control
conditions and after exposure to tumor necrosis factor has been performed. We have found that in such treatment
there is a maximum effect after 48 h, which is expressed in the stimulation of apoptosis and the accumulation
of cells with micronuclei and binuclear cells. The induction of premature chromosome condensation is an
early marker of the TNF influence. Changing the composition of the population by the number of chromosomes
in the cell may lead to the emergence of a subline with new properties compared to the parental cell population.
Key words: cell line U-937, TNF, cytogenetic abnormalities
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