RESOLUTION OF SPATIAL CONSTRAINTS DURING REPLICATION
OF PERIPHERAL CHROMATIN
O.A. Zhironkina,1,3,* S.Yu. Kurchashova,3 A.L. Bratseva,2 V.D. Cherepanynets,1,3
O.S. Strelkova,2 A.S. Belmont,4 I. I.Kireev 3
1 Faculty of Biology, M. V. Lomonosov Moscow State University,
2 Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University,
3 A. N. Belozersky Institute of Physical and Chemical Biology, M. V. Lomonosov Moscow State University, Russia,
and 4 University of Illinois, Urbana-Champaign, Illinois, USA;
* e-mail: ekfetisova@gmail.com
Tight association of peripheral chromatin with nuclear lamina unavoidably creates topological constraints
during replication. Additional complications are associated with high stability of lamina meshwork, which may
hinder an access of replication factors to the sites of DNA synthesis in highly condensed template with limited
mobility. In the current work we studied structural organization and dynamics of lamina as a function of replicative
status of associated peripheral heterochromatin. The studies of molecular mobility of laminas at various
stages of S-phase in vivo and using super-resolution microscopy showed no correlation between lamina dynamics
and replicative status of attached heterochromatin. These data support the hypothesis that lamina-chromatin
interactions during S-phase are regulated at the level of adapter proteins. Ultrastructural studies have demonstrated
that temporal break of lamina-chromatin connections during replication does not cause noticeable
spatial separation of replicating domains from nuclear periphery.
Key words: nuclear envelope, lamina, replication, FRAP, structured illumination microscopy, electron
microscopy
| Back
| Contents
| Main |
|
