MITOCHONDRIA-TARGETED ANTIOXIDANT SkQR1 SELECTIVELY PROTECTS
MDR-NEGATIVE CELLS AGAINST IONIZING RADIATION
E.K. Fetisova,1,2,* M.M. Antoschina,3 V.D. Ńherepanynets,1 D.S. Izumov,1 I.I. Kireev,1
R.I. Kireev,1 K.G. Lyamzaev,1,2 N.I. Riabchenko,3 B.V. Chernyak,1,2 V.P. Skulachev 1,2
1 A. N. Belozersky Institute of Physico-Chemical Biology of M. V. Lomonosov Moscow State University,
2 Mitoengineering Institute of M. V. Lomonosov Moscow State University
and 1 Federal State Institution «Medical Radiological Research Center», Ministry of Healthcare of the Russian Federation, Obninsk;
* e-mail: ekfetisova@gmail.com
Radioprotection appeared to be an important problem of today due to atom energetic development and utilization
of radiation material in the industry, science and medicine. It has been shown that mitochondrial targeted
antioxidant SkQR1 could attenuate radiation injury of human erythroleukemia K562 cells. Pretreatment
with SkQR1 before irradiation decreased DNA double strand breaks formation, diminished the number of chromosomal
aberrations and suppressed delayed ROS production. Prevention of oxidative stress and normalization
of mitochondrial function by mitochondria-targeted antioxidants may be a potential therapeutic strategy not only
against immediate consequences of radiation, but, either against its late consequences such as genomic instability.
SkQR1 did not protect against radiation-induced damage the K562 subline with high level of multidrug
resistance (MDR) due to SkQR1 extrusion with Pgp 170 MDR pump. We suggest that mitochondria-targeted
antioxidants might be used for selective protection of normal cells against radiation-induced damage without
interference with radiotherapy of MDR-positive tumors.
Key words: antioxidants, chromosomal aberrations, double strand breaks (DSB), mitochondria, multidrug
resistance (MDR), radioprotection, reactive oxygen species (ROS)
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