EFFECTS OF LOCAL INDUCTION OF IPT-GENE IN ROOTS ON CYTOKININS CONTENT
IN LEAF CELLS TOBACCO PLANTS
L.B. Vysotskaya,1 G.R. Akhiyarova,1 G.V. Sharipova,1 M.V. Dedova,1 S.Yu. Veselov,2
D.Yu. Zaytsev,1 G.R. Kudoyarova 1
1 Institute of Biology, Ufa Seientific Center RAS,
and 2 Department of Biology, Bashkirian State University, Ufa;
e-mail: Vysotskaya@anrb.ru
Identification of cytokinins in differentiated leaf cells has received little attention. We have carried out immunohistochemical
localization of cytokinins in leaves of transgenic tobacco plants in which the level of increased
due to induced in their roots the expression of ipt-gene controlling cytokinin synthesis. Immuno-labeling
of cytokinins with the help of antibodies raised against zeatin riboside was characteristic of mesophyll cells.
The label was localized in cytoplasm adjacent to cell walls and was absent in vacuoles. Immunohistochemical
staining also revealed the presence of cytokinins in guard cells. Induction of cytokinin synthesis enhanced the
immunohistochemical staining of both mesophyll cells and guard cells, which was accompanied by elevated
stomatal conductance. The possibility of a direct effect of cytokinins on stomatal conductance and their indirect
influence through photosynthesis in the mesophyll cells is discussed.
Key words: Nicotiana tabacum, cytokinins, immunolocalization, stomatal conductance
| Back
| Contents
| Main |
|
