IMMUNOCHEMICAL STUDY OF NUCLEAR MATRIX PROTEINS
LOCALIZATION IN THE STRUCTURE OF PERINUCLEOLAR CHROMATIN
M.I. Murasheva,1 Yu.S. Chentsov
Biology Department of M. V. Lomonosov Moscow State University;
1 e-mail: marinak-m@mail.ru,
yuchentsov@mail.ru
Immunofluorescence labeling of proteins with molecular mass of 27, 38 , 40, 50 and 65 kDa obtained from
serum of patients with autoimmune disease demonstrated different patterns (small clusters or granules) in interphase
nuclei of pig kidney cells. It was remarkable that there was no staining inside the nucleoli, but the proteins
immunoreactivity was detected around them in the regions of perinucleolar chromatin. Moreover, expression
of nucleolar proteins, such as fibrillarin and B23, was found only in nucleoli. After extraction of DNA, PNA
and histones, the proteins with molecular mass 27 and 38 kDa were found in the periphery of residual nucleoli,
and proteins with molecular mass 40, 50 and 65 kDa had similar localization and were also present in karyoplasm
of cells as small clusters. According to our data, nucleolar protein, fibrillarin, was distributed regularly
throughout the whole volume of residual nucleoli. At the same time, B23 protein was revealed only at their periphery,
where perinucleolar chromatin had localized before treatment. Thus, it has been revealed that the proteins
of nuclear matrix with molecular mass 27, 38, 40, 50 and 65 kDa, as well as nucleolar protein B23 are the
parts of perinucleolar chromatin, which could be considered as special chromosomal domain associated with
the functioning of the nucleolus.
Key words: nucleus, nucleolus, chromatin, nuclear protein matrix, residual nucleolus, immunochemistry
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