THE PROBLEM OF GENOMIC INSTABILITY OF CULTIVATED HUMAN STEM CELLS
G.G. Poljanskaya
Institute of Cytology RAS, St. Petersburg;
e-mail: poljansk@mail.cytspb.rssi.ru
The wide application of human stem cells in biomedical technologies leads to the necessity to analyze the
genomic stability of cultivated stem cells of different origin.The review presents data on genetically stable and
unstable continuous lines of human embryonic stem cells (hESC), their differentiated derivatives and adult
stem cells. Causes of genomic instability occurrence are considered and analysis of the methods used to study
the genome of celllines has been carried out. The results presented in this review demonstrate the need for periodic
control of genomic instability of all types of stem cells. Molecular-genetic analysis in order to avoid contamination
of adult stem cell lines by tumor cells is also needed. This aspect is particularly important in connection
with the active use of the adult stem cells for regenerative medicine.
Key words: embryonic stem cells, mesenchymal stem cells, neural stem cells, telomere, karyotypic
structure, genomic instability
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