VITAL MEASUREMENT OF OPTICAL DENSITY OF HELA CELL LINE
Yu.P. Petrov,1 E.S. Bozhokina, N.V. Tsupkina
Institute of Cytology RAS, St. Petersburg;
1 e-mail: yupe3ov@mail.ru
Light absorption by the live intact HeLa cells during light microscopy was studied. The light absorption may be considered as a parameter analogous to optical density used in
spectrophotometry. This parameter can be used as a quantitative characteristic of life cell as well as intracellular structures. It is shown that cells from one population but belonging
to two different clones were differed by their optical density. Optical density correlation between the shadow peripheral regions of the cells and a actin localization in these regions
was established.
Key words: live cells, light microscopy, optical density
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