STIMULATION OF CELL CULTURES RECOVERY AFTER CRYOPRESERVATION BY THE CATTLE CORD BLOOD FRACTION (BELOW 5 kDa)
OR ACTOVEGIN
A.K. Gulevsky, A.V. Trifonova, A.A. Lavrik
Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkov, Ukraine;
e-mail: trifonova_ann@rambler.ru
The capacities of the cattle cord blood low-molecular fraction (below 5 kDa) and Actovegin (the vealer blood fraction (below 5 kDa)) for recovering functions of cell cultures
after cryopreservation compared. Their influence proliferation of the flozen-thawed cell cultures, certain stages of their growth, cell attachment, rate of cell spreading, and mitotic
regiment has been studied. Both the cord blood low-molecular fraction and Actove-gin were shown to stimulate growth of the cell cultures after cryopreservation more efficiently at the
concentration of 224 mg/ml. However, despite the stimulating effect discovered, their application did not bring prolifera-tive indices on the 1st passage after cryopreservation
to the values of the native culture. The effects of the cord blood low-molecular fraction and Actovegin on the human fibroblast culture were identical by the following parameters: cell
attachment, rates of cell spreading and proliferation. In culture BHK-21 clone 13/04 the efficiency of Actovegin was low, while the cord blood low-molecular fraction has a conspicuous
stimulating effect on its adhesion and proliferation. The investigations carried out can serve as a basis for the development of regenerative media containing the cattle cord blood
low-molecular fraction (below 5 kDa) or Actovegin as active components at the concentration of 224 mg/ml with the purpose of fast recovery of culture prolifetative properties after
cryopreservation.
Key words: cryopreservation, cattle cord blood fraction (below 5 kDa), Actovegin, cell cultures, recovery, proliferation, adhesion, mitotic regimen
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