COMPARATIVE ANALYSIS OF THE EXOGENOUS AMMONIUM INFLUENCE ON SOYBEAN TISSUE AND CHLAMYDOMONAS CELLS
A.P. Smolov,1,* A.M. Butanaev,1 G.A. Semenova,2 G.N. Shirshikova 1
1 Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya ul.2, Pushchino, Moscow oblast, 142290 Russia,
2 Institute of Theoretical and Experimental Biophysics Russian Academy of Sciences, Institutskaya ul.3, Pushchino, Moscow oblast, 142290 Russia;
* e-mail: smap49@mail.ru
In this work we studied the influence of exogenous ammonium on the total protein and chlorophyll contents, on the number of ribosomes and on the expression of ribosomal genes
encoding the small subunit 18S rRNA and rpS6 protein in unicellular green alga, Chlamydomonas reinhardtii, and in callus tissue of Glycine max. Comparative analysis
of two sets of data showed that although the lack of ammonium resulted in reduction of the number of ribosomes in alga and plant cells, this effect was not caused by decreasing of the
expression level of the ribosomal genes. Possible mechanisms of the ammonium regulatory role in the ribosome biogenesis are discussed.
Key words: callus soybean, chlamydomonas, ammonium, ribosomes, ribosomal genes
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