CELLS OF ENDOTHELIAL LINEAGE (OR ENDOTHELIAL-LIKE CELLS) AS POSSIBLE PROGENITOR CELLS OF SARCOMAS INDUCED BY IMPLANTED
FOREIGN BODY
T.G. Moizhess,* Ju.M. Vasiliev
N.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences;
* e-mail: kor@physiol.ru
This study is dedicated to the problem of the origin of progenitor cells of sarcomas induced by implantation to mice of foreign body (polyvinilchloride plates). It is known that these
cells are found among the cells of the monolayer covering the surface of implanted plate without any signs of tumor growth in 6 and more months after implantation. We studied the
existence of endothelial features of mesenchymal type cells that was grown by culturing the cells from the implanted plate surface in 9 and 14 months after implantation. It was shown
in this work that these cells are really the precursors of FB sarcomas thou they had different tumor potential depending on the period since implantation moment. We studied the behavior
of these pretumor cells in 2D (on plastic) and 3D (on matrigel) cultures. We have shown that such cells in dense monolayer on plastic gave growth of endothelial type (cobblestone).
When sown on matrigel they was organized in more or less typical capillary-like structures that subsequently formed secondary branching vascular-like structures, so named "secondary
sprouting". Such behavior is also characteristic for endothelial cells. This observations allow us to conclude on possibility of endothelial origin of FB sarcomas.
Key words: FB carcinogenesis, FB sarcomas, matrigel, endothelial cells
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