THRESHOLD RAT NEONATAL CARIOMYCYTE RESPONSE TO GRADUAL CRYPTOSPORIDAL INFECTION SEVERITY INCREASE
O.V Anatskaya,* N.V. Sidorenko, I.V. Matveev, A.V. Kropotov, M.V. Kharchenko, A.E. Vinogradov
Institute of Cytology RAS, St. Petersburg;
* e-mail: springwater@mail.ru
Infectious gastroenteritis is one of the common causes of tachyarytmia, malabsorbtion and growth retardation in children. Our recent studies indicated that neonatal cryptosporidial
gastroenteritis is associated with long-term cardiomyocyte abnormalities. The aim of the present study was to find out how neonatal cryptosporidiosis of various severities affects cardiac
anatomy and cardiomyocyte polyploidization, remodeling and HIF-1α expression. Using real-time PCR, cytometry, immunohistochemistry, image analysis and interatrial septum
visual examination, we revealed that gradual increase in cryptosporidial invasion was associated with threshold changes. At weak parasitic infection, interatrial septum was entire and
there were no statistically significant changes in cardiomyocytes. At moderate and severe infection, all changes in cardiac anatomy and cardiomyocytes were statistically significant and
demonstrated approximately similar degree. Compared to control, heart were atrophied and elongated, interatrial septum contained a small window (patent foramen ovale), and
cardiomyocytes lost protein, became elongated, thin and accumulated additional genomes. Also we found HIF-1α mRNA hyperexpression. Notable, the threshold response to
gradual stimulus is an important criterion of developmental programming since such a response is commonly a consequence of abnormal anatomic structure formation and cell differentiation
failure. Our results can be interesting for physicians because they indicate that even moderate cryptosporidiosis can be dangerous for neonatal heart and can trigger neonatal programming
of cardiovascular pathology. Also, our results for the first time demonstrate the association between gastroenteritis, patent foramen ovale and cadriomyocyte malfunction.
Key words: neonatal cardiomyocyte, cryptosporidial gastroenteritis, polyploidy, remodeling, interatrial septum, foramen ovale, HIF-1α,
developmental programming
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