LINE CLASS RETROPOSON IS THE COMPONENT OF THE DNA POLYMORPHIC FRAGMENTS PATTERN OF TREMATODE HIMASTHLA ELONGATA PARTHENITAE
A.I. Solovyeva,1,2 N.K. Galaktionov,1 O.I. Podgornaya 1,2
1 Faculty of Biology and Soil Sciences of St. Petersburg State University and
2 Institute of Cytology RAS, St. Petersburg;
e-mail: orcinuca@gmail.com
We have determined that S-SAP method (Sequence specific amplification polymorphism) reveals clonal variability in the genomes of larvae of flatworm Himasthla elongata
(Trematoda, Echinostomatidae). Being parthenogenetic the larvae were previously considered to be genetically homogeneous. Cloning and sequencing of a ~500 bp conservative fragment
(B1) from the fragments' pattern has been performed. Sequence analysis of B1 has shown that this fragment has maximum homology with LINE elements from CR1 family of Hydra and sparrow.
In situ hybridization (FISH) has detected dispersed distribution of B1. Several other fragments cloned from the same lane of agarose electrophoresis correspond to conservative domain
of reverse transcriptase (RT) from CR1 family. Thus, we have shown that 1) cercariae of trematode H. elongata have clonal variability; 2) the S-SAP method allows to obtaining patterns
of fragment distribution characteristic of individual cercariae; 3) conservative domain of RT of CR1 family participates in the pattern of polymorphic fragments generation. Identification of the
CR1 transcripts in cercariae of H. elongata transcriptome is the aim of the future work. Cloning of the variable fragments from the fragments' pattern is in progress.
Key words: LINE, CR1, S-SAP, clonal variability, flatworms
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