INTERPOPULATION DEFFERENCES IN PARAMETERS OF HEMOCYTE DNA-COMETS OF SNAIL LYMNAEA STAGNALIS FROM REGIONS WITH THE DIFFERENT
ENVIRONMENTAL LOAD
A.Yu. Koneva
Institute of Genetics and Cytology of the NAS of Belarus, Minsk;
e-mail: koneva@tut.by
The research of hemocytes of snail Lymnaea stagnalis from regions with different environmental load has been carried out by means of DNA-comet assay. Significant
interpopulation distinctions in parameters of hemocytes DNA comets, and also significant differences of semitivity of hemocyte genetic matherial in snails form different ecological
zones to the influence of external damaging factors (in particular, heavy metals) have been revealed by means of the software analysis of hemocyte DNA-comet images. Since the
two populations of mollusks are characterized by high genetic identity, the different levels of proliferative processes in hemocytes of snail Lymnaea stagnalis from different
ecological zones (that we revealed using the comet assay) may act as an indicator of the intensity of damaging effects and environmental quality.
Key words: DNA-comet, hemocyte, Lymnaea stagnalis populations, polyploidy, polyteny, adaptation, bioindication, environmental load
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