CHARACTERISTICS OF PRIMARY CELL CULTURE FROM NEONATAL THYROID GRAND OF PIGS: FOLLICULOGENESIS, HORMONE AND GROWTH
S.B. Bilyavskaya,1 G.A. Bozhok,1 E.I. Legach,1 I.A. Borovoy,2
I.M. Gella,2 Yu.V. Malyukin,2 T.P. Bondarenko 1
1 Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine
and 2 Institute for Scintillation Materials NAS of Ukraine, Kharkov;
e-mail: s_bilyavskaya@mail.ru
In the study, comprehensive assessment of proliferative and hormonal activity of primary cell cultures derived from neonatal pig thyroid has been carried out for the first time.
We have evaluated the basal and TSH-stimulated secretion of thyroxine and morphological features of culture, depending on the initial state of the material placed in culture:
in the form of single cells or follicular conglomerates. Folliculogenesis and formation of dome structures were observed in culture spontaneous and under chronic TSH stimulation.
The ability of the cells to expression of β-III-tubulin during prolonged cultivation in the presence of NGF has been demonstrated in the study.
Key words: thyroid gland, primary culture, follicle, thyrocyte, thyroxine, thyroid-stimulating hormone
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