DROSOPHILA MELANOGASTER IMAGINAL DISCS MITOTIC ANOMALIES INDUCED BY TUMOR-SUPRESSOR Dlg SILENSING CONSTRUCTION
L.I. Lebedeva,1 T.D. Dubatolova,1 L.V. Omelyanchuk 1,2
1 Institute of Molecular and Cellular Biology SB RAS, Novosibirsk and 2 Novosibirsk University;
e-mail: ome@mcb.nsc.ru
Mitosis, cytokinesis and nuclear texture of wing imaginal discs cells silenced by UAS-RNAi-dlg construct induced by 1096-Gal4 driver were studied. The silencing construct
contains coding region of dlg gene and the complementary region. Further this RNA hairpin (Dietzl et al., 2007) is processed by endogenous protein Dicer and the resulting RNA
fragments silence mRNA dlg. Tumor suppressor gene dlg is coding for 21 transcripts. The construct UAS-RNAi-dlg inactivates 14 transcripts - RE, RH, RQ, RS, RG, RD, RL, RB,
RK, RR, RT, RN, RA, RP - and do not silenced 7 others (RO, RF, RI, RU, RJ, RC, RM). This permits to study the fuction of proteins containg guanilate-kinase domain IPR008145 at
C-end of the protein. Most important consequences of the silencing is the abnormal mitosis exit and binuclear cells formation. Quantitative fluorescence measurements of anti-Í3-ð
histone and DAPI signals showed phase-specific changes in nuclear texture. The most probable dlg target of in mitosis is the inactivation of cellular cortex polarization.
Key words: Dlg, UAS-RNAi-dlg, 1096-Gal4 driver, confocal microscopy, mitosis, nuclear texture
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