A COMPARATIVE ANALYSIS OF COLORECTAL CARCINOMA CELL LINES THAT DIFFER IN METASTATIC POTENTIAL
A.P. Davydov-Sinitsyn,1 O.V. Bajenova,2 M.A. Liskovykh,1 S.V. Ponomartsev,1
L.L. Chechik,1 A.N. Tomilin,1 E.N. Tolkunova 1
1 Institute of Cytology of the RAS, Saint Petersburg and 2 Saint Petersburg State University;
e-mail: ads707@ya.ru
In the current work we make an attempt to compare cancer cells of one origin, but differing in the expression of CEA protein - a clinical marker of metastatic carcinomas,
presumably one of the key factors in metastatic activity. We explore the morphology of cell colonies in vitro, expression patterns of epithelial markers, the ability of these cells to
form tumors and metastases in vivo, and evaluate their stem compartment with the aid of a suicidal genetic construct sensitive to an embryonic stem cell marker, Oct4.
Key words: cancer stem cells, tumorigenicity, Oct4, POU5F1
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