ABILITY OF THE INFLUENZA VIRUSES AND THEIR ENVELOPE PROTEINS TO STIMULATES ENDOTHELIAL CELLS APOPTOSIS IN VITRO
Azarenok A.A.,1 Prochyhanova A.R.,1 Zenin V.V.,2 Lyublinskaya O.G.,2
Kozlova N.M.,1 Zhilinskaya. I.N. 1
1 Research Institute of Influenza and 2 Institute of Cytology RAS, Saint-Petersburg;
e-mail: irina@influenza.spb.ru
The ability was studied of the modern epidemic strains of influenza virus type A (subtypes H5N1, H3N2, H1N1pdm) and their surface proteins, hemagglutinin (HA) and
neuraminidase, to cause the activation of the cellular protein caspase-3 and stimulate the emergence of phosphatidylserine on the membrane of human endothelial cell line EAhy926.
It was questioned how the viruses and their surface proteins that were studied cause the activation of caspase-3 after 0.5 hours of exposure that recorded immunogistotsitohimicheski.
The test viruses and neuraminidase (concentration 10mkg/ml) led to the appearance of phosphatidylserine on the cell membrane in a time interval of 2-8 hours from the beginning of the
treatment, which was recorded by flow cytometry. The death of endothelial cells when exposed to the HA (in a concentration of 50 ug / ml) and in the same time frame was not
accompanied by the appearance of phosphatidylserine. The specific features of apoptotic cell death during the reproduction of the virus are described, as well as the effects of viral
proteins.
Key words: influenza virus, hemagglutinin, neuraminidase, apoptosis, caspase-3
| Back
| Contents
| Main |
|
