EFFECT OF HYDROSTATIC PRESSURE ON STRUCTURAL ORGANIZATION OF THE MAIZE ROOT CELLS
F.A. Abdrakhimov, M.A. Suslov, A.V. Anisimov
Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences;
e-mail: abdfar@mail.ru
We analyzed the effect of hydrostatic pressure at 2-4 MPa level on growth and organization of corn seedlings cells. At pressure value of 2 MPa we observed the clarification of
mitochondrial matrix, clusterization of endoplasmic reticulum elements with formation of ergastoplasm sites, and increasing number of small vacuoles in the cytoplasm. On the trans side
of dictyosomes we detected the accumulation of vesicles with electron-dense content which points to traffic changes in endomembranous system. The reduction of electronic density
of tonoplast was accompanied with reduction of osmiophilic inclusions quantity in vacuoles. Increase of pressure to 4 MPa has shown the occurrence of destructive alterations in the
tonoplast. None of the variants showed any violation of plasmalemma integrity, confirmed by coloring with Evans blue. When dumping the pressure to atmospheric value the frequency
of structural distortions of the tonoplast membrane was proportional to pressure difference: minimum at dump from 2 to 0 MPa and maximum at dump from 4 to 0 MPa. Thus it was
concluded, that the endomembraneous system is among the targets of pressure effect on the plant cells.
Key words: pressure, stress, endoplasmic reticulum, corn seedlings
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