DETECTION OF THE REPLICATION SITES IN PLANT CELL NUCLEI USING SEMITHIN SECTIONS
Kuznetsova M.A.,1 Sheval E.V. 2
1 Department of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow and
2Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;
e-mail: sheval_e@genebee.msu.su
A novel approach for the detection of replication sites in plant cells nuclei is described. Included nucleotide (EdU) was detected using "click"-chemistry in semithin sections of the
material embedded in acrylic resin. The usage of the protocol introduced allows: (1) to safe the intact morphology of cells, (2) to work with any tissue, and (3) to obtain high-resolution
during microscopy (especially, axial).
Key words: nuclei, chromatin, replication, replication sites, EdU, semithin sections
| Back
| Contents
| Main |
