EFFECT OF CELL MICROENVIRONMENT ON CELL FUNCTION ASSOCIATED WITH TUMOUR PROMOTION AND PROGRESSION
I.I. Koldin,1,2 E.M. Trechalina,2 Yu.Yu. Sharovskaya,3 T.K. Dubovaya,1 V.A. Kobliakov 2
1 N.I. Pyrogov Russian State Medical University, 2 N.N. Blochin Russian Cancer research Centre RAMS,
3 A.N. Belozersky Institute of Physico-Chemical Biology of Moscow State University; Moscow;
e-mail: kobliakov@rambler.ru
For the investigation of tumour-promotion activity of cell environment the transformed embryonic rat fibroblasts (clone CL-1) were transfect to immunodeficit mice and formed
tumour cells were cultivated (cells CL-1-1). The cells before and after transplantation were compared by morphology, proliferation activity and gap junction intercellular communications.
The CL-1-1 cells proliferated much quicker, than CL-1 cells, have the changed morphology structure and in CL-1-1 unlike CL-1 the contact inhibition was absent. In phase
G1 of cell cycle the CL-1 cells were much more than CL-1-1, whereas in G2/M phases the cells CL-1-1 were much more than CL-1 ones. The activity of
gap junction intercellular communications in both cell types was near the same. It was concluded that by cell transplantation to immunodeficit mice the cell microenvironment act as a
tumour-promoter and tumour progression factor.
Key words: tumour promotion, tumour progression, proliferation, gap junction intercellular communications
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