ABILITY OF SU(HW) TO CREATE A PLATFORM FOR ORC BINDING DOES NOT DEPEND ON TYPE OF SURROUNDING CHROMATIN
M.U. Mazina, N.E. Vorobyeva, A.N. Krasnov
Institute of Gene Biology RAS, Moscow;
e-mail: krasnov@genebiology.ru
DNA replication begins from multiple sites distributed throughout the genome, named replication origins. Despite the increasing amount of data on the properties of replication origins,
it is still unknown what factor(s) is the primary determinant of ORC localization. Su(Hw) is a zinc-finger protein that is responsible for the activity of the best-studied Drosophila
insulators. Here, we show that insulator protein Su(Hw) recruits histone acetyltransferase complex SAGA and chromatin remodeler dSWI/SNF to Su(Hw)-dependent insulators and
creates platform for ORC binding. We found that Su(Hw) is necessary for chromatin remodeling and ORC recruitment regardless of type of surrounding chromatin. Thus, global
chromatin state does not influence molecular mechanism underlying ORC positioning in the genome, rather DNA-binding proteins are key determinants which create proper chromatin
structure for ORC binding. Su(Hw) is the first example of such a protein.
Key words: Su(Hw) protein, replication origins, ORC, dSWI/SNF, SAGA
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