NOVEL COMPLEXES OF GENE EXPRESSION AND THEIR ROLE IN THE APPEARANCE AND EVOLUTION OF THE GENUS HOMO
E.K. Shematorova, D.G. Shpakovski, G.V. Shpakovski
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow;
e-mail: gvs@ibch.ru
With use of genetic (yeast two-hybrid system) and biochemical (co-precipitation of proteins from cellular lysates) approaches we have performed a whole-genome wide search
for interacting partners of the previously described by us variants of hRPB11 subunit of human RNA polymerase II - hRPB11bα, hRPB11ñα and hRPB11bβ,
hRPB11cβ - in fetal brain and Jurkat cell line libraries. In consequence, the main spectrum of the protein partners of these human specific isoforms of the RNA polymerase
II subunit hRPB11 (POLR2J) was established. Functional characteristics of the uncovered protein partners of hRPB11bα and hRPB11ñα isoforms clearly indicate that these
isoforms, similarly to the main (major) subunit hRPB11a, are components of the distinct transcription complexes participating not only in the transcription of the specific DNA matrices,
but involving also in the later stages of mRNA biogenesis. The RNA polymerase II subunit hRPB6 (POLR2F) and basal component of the exon-exon junction complex Y14 (RBM8A)
were found among protein partners of the isoforms hRPB11bβ and hRPB11cβ, together with number of proteins participating in biogenesis of microRNAs including novel,
previously undescribed variant of the microRNA processing nuclease DROSHA which points out on the existence of special coordination between processes of transcription and RNA
interference in nuclei of human cells.
Key words: segmental duplications, human POLR2J gene family, minor isoforms of human RNA polymerase II subunit hRPB11 (POLR2J), yeast two-hybrid system,
protein partners, COMMD4d, evolution of Homo sapiens
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