NEUROGENIC POTENTIAL OF HUMAN MESENCHYMAL STEM CELLS ISOLATED FROM BONE MARROW, ADIPOSE TISSUE AND ENDOMETRIUM:
A COMPARATIVE STUDY
V.I. Zemelko,2 I.B. Kozhucharova,1,2 L.L. Alekseenko,2.A.P. Domnina,2
G.F. Reshetnikova,1,2 M.V.Puzanov,1 R.I. Dmitrieva,1
T.M. Grinchuk,1,2 N.N. Nikolskiy,2 S.V. Anisimov 1,2
1 V.A. Almazov Federal Center for Heart, Blood and Endocrinology, Saint-Petersburg, and
2 Institute of Cytology RA S, Saint-Petersburg;
e-mail: vzemelko@mail.ru
Mesenchymal stem cells (MSCs) can be isolated from many adult tissue sources. These cells are a valuable substrate in cell therapy for many diseases and injuries.
Different types of MSCs vary in plasticity. We performed a comparative study of the neurogenic potential of three types of human MSCs derived from bone marrow (BMSCs),
subcutaneous adipose tissue (ADSCs) and endometrium (isolated from the menstrual blood) (åMSCs). It was shown that all three types of MSC cultures demonstrate multipotent
plasticity and predisposition to neurogenesis, based on the expression of pluripotency marker SSEA-4 and neuronal precursors markers nestin and beta-III-tubulin. Further analysis
revealed a transcription of the neuronal marker MAP2 and neurotrophin-3 in undifferentiated BMSCs and ADSCs. Additionally, a significant basal level of synthesis of brain-derived
neurotrophic factor (BDNF) in åMSC culture was also observed. Stimulation of neural induction with agents such as 5-azacytidine, recombinant human basic fibroblast growth factor
(bFGF), recombinant human epidermal growth factor (EGF), a recombinant human fibroblast growth factor 8 (FGF8), morphogen SHH (sonic hedgehog), retinoic acid (RA) and
isobutyl-methyl-xanthine (IBMX), showed further differences in the neurogenic potential of the MSCs. The components of the extracellular matrix, such as Matrigel and laminin,
were also the important inducers of differentiation. The most effective neural induction in BMSCs proceeded without the RA participation while pretreated with 5-azacytidine.
In contrary, in case of åMSCs RA was a necessary agent of neural differentiation as it stimulated the transcription of neurotrophin-4 and the elevation of secretion level of BDNF.
The use of laminin as the substrate in EMSCs appeared to be critical, though an incubation of the cells with 5-azacytidine was optional. As far as ADSCs, RA in combination with
5-azacytidine causes the elevation of expression of MAP2, but reduced the secretion of BDNF. Thus, the effect of RA on neural differentiation of ADSCs is ambiguous and,
together with the study of its signaling pathways in the MSCs, requires further research. The therapeutic effect of transplanted MSCs is commonly explained by their paracrine
activity. The high basal level of BDNF synthesis in the åMSCs, along with their high proliferative rate, non-invasive extraction and neural predisposition, is a powerful argument
for the use of the intact EMSCs as a substrate in cell therapy to repair a nerve tissue.
Key words: cell therapy, mesenchymal stem cells
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