THE EFFECTS OF RH421 ON THE ACTIVITY OF AMPHOTERICIN B IN CELL AND MODEL MEMBRANES
E.V. Mikhailova,* S.S. Efimova, O.S. Ostroumova
Institute of cytology RAS, St. Petersburg;
* e-mail: mikhailovaev@list.ru
The combined action of polyene macrolide antibiotic amphotericin B and styryl dye RH 421 in model lipid membranes and in yeast Saccharomyces cerevisiae is studied.
RH 421 insertion into the ergosterol-containing lipid bilayers leads to the increase of the steady-state number of open amphotericin channels by 8.5 ± 3.2 times. Paper disk agar
diffusion method demonstrates that RH 421 enhances the antimicrobial effect of amphotericin B leading to the increase of zone of growth inhibition of S. cerevisiae strain by 1.5 times.
Our data suggest that RH 421 is a potential synergist of amphotericin B and can be used in new formulations with improved pharmacological properties.
Key words: amphotericin B, RH 421, lipid bilayers, Saccharomyces cerevisiae
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