TSPO LIGAND PK11195 AND MAPK INHIBITOR UO126 MODULATE TSPO EXPRESSION LEVEL
S.N. Gyrylova, T.G. Ruksha, A.V. Komina
Krasnoyarsk State Medical University named after prof. V.F.Voino-Yasenetski;
e-mail: sveta_sol84@mail.ru
Translocator protein TSPO and MAPK signaling pathway are important regulators of numerous cell functions, including carcinogenesis, cell proliferation and apoptosis.
We have studied MAPK inhibitor UO126 and TSPO specific ligand PK 11195 effects on TSPO expression level in melanoma cells. Using immunocytochemichal staining we have
detected increase of TSPO expression after incubation with PK11195 and UO126 in all concentrations. These results were confirmed by real time PCR: it was detected increase of
mRNA TSPO expression level after incubation with PK11195 in concentration 100 nmol/L, and with UO126 in concentration 10 ?mol/L. In case of combination treatment with PK11195
and UO126 it was also increase of TSPO expression level. In conclusion TSPO ligand PK11195 and MAPK signaling pathway inhibitor UO126 modulate TSPO expression. These data
are crucial for indentifying of regulatory processes for TSPO expression. Pathogenetic interconnection between MAPK signaling pathway and TSPO is important for novel therapeutic
approaches in melanoma treatment.
Key words: adenylyl cyclase, adrenergic receptor, guanylyl cyclase, natriuretic peptide, motility, spermatozoa
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