Tsitologiya  2013  55 (12) : 893–900
MORPHOLOGICAL ANALYSIS OF HEMOCYTES OF ASCIDIAN HALOCYNTHIA AURANTIUM

A.N. Sukhachev,1 I.S. Dyatchkov,1 D.S. Romanyuk,1 V.V. Kumeyko,3,4 V.F. Sinitsina,2
E.D. Korolkova,2 A.D. Kharazova,2 A.V. Polevschikov 1-3,*

1 Institute of Experimental Medicine of North-West Branch of RAMS, St. Petersburg, 2 Department of Cytology and Histology, St. Petersburg State University, 3 Biomedical School, Far Eastern Federal University
and 4 A. V. Zhirmunsky Institute of Marine Biology RAS, Vladivostok;
* e-mail: alexpol512@yandex.ru

The current paper is devoted to the investigation of ascidian circulating hemocytes populations which are used as an example of blood cell specialization in the lower Chordates. Work has been performed on a solitary ascidian Halocynthis aurantium from the Japanese Sea. Using light microscopy and histiochemistry, we have identified five main populations of circulating hemocytes (hemoblasts, granulocytes, hyaline amoebocytes, macrophage-like and morula cells), which are typical of all tunicates. Pigment cells were not revealed. The possible pathway of circulating cells differentiation is assumed.

Key words:  ascidia, hemocytes, morphological analysis


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