LONG-TERM EXPANSION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS UNDER REDUCED OXYGEN TENSION
Yu.V. Rylova, L.B. Buravkova
Institute of Biomedical Problems RAS, Moscow;
e-mail: yuliaril@mail.ru
We have shown that the decrease in oxygen tension in the culture medium of multipotent mesenchymal stromal cells (MMSCs) results in a short-term reduction in the proportion of
CD73+-cells in the population, wit-hout effecting the number of cells expressing other constitutive surface markers (CD90 and CD105). In this case, the heterogeneity of
the cell population declined: large spread cells disappeared. The proliferative activity of MMSCs significantly increased and remained stable in conditions in which the oxygen content
was close to the tissue oxygen levels (5 % O2). At lower oxygen concentration, proliferative activity of the cells gradually reduced from passages 3-4. The increase in
proliferative activity was not accompanied by increased expression of telomerase gene indicateding the alsance of cell transformation. However, genome-wide analysis of MMSC
gene expression level revealed changes in expression of cyclins (CCND2 and PCNA), regulatory subunit cyc-lin-dependent kinase (CKS2) and an inhibitor of cyclin-dependent
kinase (CDKN2C), regulating the cell cycle, which is obviously facilitated the increase in the proliferative capacity of cells at lower oxygen tension.
Key words: multipotent mesenchymal stromal cells, immunophenotype, morphology, proliferation
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