Tsitologiya  2013  55 (12) : 868–873
ALLOCATION OF PRECURSOR GERMINAL CELLS FROM THE TISSUE OF THE HUMAN OVARIES

A.M. Polstyanoy,1 Yu.I. Sheina, A.V. Eremeev, G.N. Polstyanaya, A.V. Svetlakov

Center of Reproductive Medicine, Krasnoyarsk;
1 e-mail: ivf@inbox.ru

The article present the results of a study on the allocation of precursor germinal cells from tissue of human ovaries obtained during various gynecological operations. Two series of cell culture were isolated from tissue of tunica albuginea. The cell lines were identified and characterized by PCR012-method, immunocytochemical staining and histological examination. The study provided data reliably confirming the presence of precursor cells in the ovarian tissue of women in their reproductive years.

Key words:  cell culture, reproductology, germinal cells, ovary, immunocytochemistry


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