HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL LOCALIZATION OF CHOLINE ACETYLTRANSFERASE IN THE NUCLEI OBLONGATA RAT BRAIN
A.E. Kotsyuba, V.M. Chertok 1
Pacific State Medical University, Vladivostok;
1 e-mail: chertokv@mail.ru
ChAT-positive neurons in the nuclei of the medulla oblongata of Wistar rats have been studied with the use of histochemical and immunohistochemical methods. We have found that the
topography and the number of cholinergic neurons at the projection nuclei studied largely depend on the method of detection of these neurons. Histochemical method always revealed more
neurons than immunohistochemistry. Such a feature of ChAT-po-sitive neurons detection was clearly seen among the majority of nuclei in the medial region and in some of the nuclei in the
lateral region of the medulla oblongata. The number of immunoreactive cells in the nuclei varied from 17 to 26 %, whereas the histochemical reaction determined 1.5-3 times more neurons in the
same nuclei. ChAT-positive cells in the nuclei of the back seam were detected mainly by a histochemical method.
Key words: cholinergic neurons, the nucleus of the medulla oblongata
| Back
| Contents
| Main |
