COMPARATIVE ANTIOXIDANT ACTION ON THE LEVEL OF REACTIVE OXYGEN SPECIES
IN NORMAL AND TRANSFORMED FIBROBLASTS
O.G. Lyublinskaya, K.M. Kirpichnikova, I.A. Gamaley 1
1 e-mail: igamaley@mail.cytspb.rssi.ru
We studied the changes in the level of reactive oxygen species (ROS) in normal (3T3) and transformed
(3T3-SV40) murine fibroblasts under the antioxidant action for 15 min using fluorescent probe carboxy-
H2DCFDA. We have shown that N-acetylcysteine (NAC) decreased ROS level in both cellular types. Another
antioxidant, alpha-lipoic acid (ALA), and its reduced form, dihydrolipoic acid (DHLA), caused the prooxidant effects.
Both ALA and DHLA in the concentration range of 0.1—1.25 mM increased ROS level in dose dependent
manner in both cellular types. The ability of ALA and DHLA to activate hydrogen peroxide production is discussed.
Key words: reactive oxygen species, N-acetylcysteine, alpha-lipoic acid, dihydrolipoic acid, prooxidant actin
| Back
| Contents
| Main |
