FUNCTIONAL PROPERTIES OF SMOOTH MUSCLE CELLS IN ASCENDING AORTIC ANEURYSM
D.A. Kostina,1 I.V. Voronkina,2 L.V. Smagina,2 N.D. Gavriliuk,1 O.M. Moiseeva,1
O.B. Irtiuga,1 V.E. Uspensky,1 A.A. Kostareva,1 A.B. Malashicheva 3,*
1 Almazov Federal Heart, Blood and Endocrinology Centre, 2 Institute of Cytology RAS, St. Petersburg, and
3 Department of Embryology, St. Petersburg State University;
* e-mail: malashicheva_ab@almazovcentre.ru
Thoracic aortic aneurism (TAA) develops as a result of complex series of events that dynamically alter the
structure and composition of the aortic vascular extracellular matrix (ECM). The main elements that alter the
composition of aortic wall are smooth muscle cells (SMC). The purpose of the present work was to study alteration
of smooth muscle cell functions derived from the patients with TAA and from healthy donors. As it is supposed
that TAA associated with bicuspid aortic valve (BAV) and with tricuspid aortic valve (TAV) differ in
their pathogenesis, we compared the SMC and tissues samples from BAV-, TAV-patients and healthy donors.
We compared TAA patients’ derived tissues and SMC to healthy donors’ ones in several parameters: SMC
growth, migration and apoptotic dynamics; metalloproteinase MMP2 and MMP9 activity (zymography) and
elastin, collagen and fibrillin content (Western blot) in both tissue samples and cultured SMC. Proliferation ability
of both BAV and TAV SMC was decreased comparing to donors cells; migration ability in scratch tests was
increased in TAV-derived SMC comparing to donor cells. BAV-cells migration ability was not changed comparing
to donor-SMC. Elastin content was decreased in TAA SMC comparing to donor cells whereas the content
of fibrillin and collagen was not altered. At the same time elastin and collagen protein level was significantly
higher in tissue samples of TAA patients comparing to donor-derived samples. SMS proliferation and migration
ability is differently affected in TAV and BAV-associated TAA that supports the idea of different nature of
these two groups of TAA. Also our data show that SMC functional properties are altered in TAA patients and
these alterations could play a significant role in the disease pathogenesis.
Key words: extracellular matrix, matrix metalloproteases, smooth muscle cells, thoracic aortic aneurysm
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