EXTRACELLULAR MATRIX DEPENDENCE OF THE CARDIOMYOCYTE CONTRACTILE APPARATUS ORGANIZATION
N.B. Bildjug,1 G.P. Pinaev
Institute of Cytology RAS, St. Petersburg;
1 e-mail: relapse@yandex.ru
In the process of cardiomyocyte culturing, their contractile apparatus undergoes reorganization involving
the convertation of typical myofibrils into non-muscle type structures and subsequent restoration of the initial
organization. The causes and mechanisms of the rearrangements described are unknown. In this study, we have
shown that cultivation of cardiomyocytes on the individual extracellular matrix proteins, as well as on the
matrix produced either by cardiac fibroblasts or by similar cardiomyocyte, reduses the time when the contractile
apparatus is in a rearranged state, whereas soluble factors of the conditioned culture do not affect obviously these
rearrangements. Using method of extracellular matrix isolation adapted for cardiomyocytes, we have shown
that cardiomyocytes in culture produce their own extracellular matrix, which differs from the extracellular matrix
of cardiac fibroblasts and varies with the culturing time.
Key words: cardiomyocytes, contractile apparatus, extracellular matrix
| Back
| Contents
| Main |
