ACTIN AND mrna EXPORT FACTORS DISTRIBUTION IN THE NUCLEUS OF PREOVULATORY MOUSE OOCYTES
G. N. Pochukalina., V. N. Parfenov
Institute of Cytology, RAS, St. Petersburg;
e-mail: galina@mail.cytspb.rssi.ru
Nuclear actin is a part of the chromatin remodeling complex involved in transcriptional and associated with nascent mRNA, providing them an ability to export from the nucleus. It is
currently proved that many factors involved in mRNA editing and mRNA export are localized in IGCs. Using confocal laser scanning microscopy and immunogols labeling technique, we
identified actin and A1 hnRNP protein in the nuclei of oocytes from mouse preovulatory follicles. We have demonstrated that nuclear actin is detected in association with the chromatin and IGCs.
Components of mRNA export complex including proteins A1 and NXF1/TAP are localized in IGCs together with actin. In accordance with the concept suggesting that IGCs participate in RNA
retention in the nucleus we discuss that mRNA transcription-export complex including actin, A1 hnRNP protein and NXF1/TAP, along with mRNA and transcription factors is formed in IGCs.
Key words: oocyte mouse nucleus, interchromatin granule clusters, actin, mRNA export, A1 A/B hnRNP, NXF1/TAP, confocal microscopy, electron microscopy
| Back
| Contents
| Main |
