COMPARISON OF HUMAN ENDOMETRIAL STEM CELLS AND FIBROBLASTS RESISTANCE TO OXIDATIVE STRESS
E. B. Burova,1 O. G. Lublinskaya, A. N. Shatrova, A. B. Borodkina, N. N. Nikolsky
Institute of Cytology of the Russian Academy of Sciences, St. Petersburg;
1 e-mail: lenbur87@mail.ru
Reaction of human endometrial stem cells (hESCs) on oxidative stress was investigated by flow cytometry. Two terminally differentiated cell lines were used for the comparison:
human embryonic lung fibroblasts and human dermal fibroblasts. The oxidative stress was designed by hydrogen peroxide (H2O2) action in the wide range of
concentrations (50-1500 µM) during 24 h. It was shown that the H2O2 amount per one cell (picomole/cell), but not H2O2
concentration in the growth medium, should be taken into account for the accurate evaluation of H2O2 effect on the different cell lines. Therefore, in our
experiments LD50 reflects the amount of H2O2 per cell under which 50% of cells survive after 24 h. We have been demonstrated that hESCs
are more resistant to H2O2 compared with embryonic lung fibroblasts, but more sensitive than dermal fibroblasts.
Key words: endometrial stem cells, H2O2, fibroblasts, LD50, oxidative stress
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