LASER MICRODISSECTION FOR BIOLOGY AND MEDICINE
O.V. Podgorny,1,2, V.N. Lasarev,1 V.M. Govorun 1
1 Insitute of Physical-Chemical Medicine, FMBA, Moscow, 2 Kot'zov Institute of Developmental Biology, Russian Academy of Sciences, Moscow;
1 e-mail: olegpodgorny@inbox.ru
For routine extraction of DNA, RNA, proteins and metabolites, small tissue pieces are placed into lysing solution. These tissue pieces in general contain different cell types.
For this reason, lysate contains components of different cell types, which complicates the interpretation of molecular analysis results. The laser microdissection allows overcoming
this trouble. The laser microdissection is a method to procure tissue samples contained defined cell subpopulations, individual cells and even subsellular components under direct
microscopic visualization. Collected samples can be undergone to different downstream molecular assays: DNA analysis, RNA transcript profiling, cDNA library generation and
gene expression analysis, proteomic analysis and metabolite profiling. The laser microdissection has wide applications in oncology (research and routine), cellular and molecular
biology, biochemistry and forensics. This paper reviews the principles of different laser microdissection instruments, examples of laser microdissection application and problems
of sample preparation for laser microdissection.
Key words: laser microdissection, methods of molecular biology and biochemistry
| Back
| Contents
| Main |
|
