THE ESTIMATION IN SIMILARITY OF CHARACTERISTICS OF THE POST-MITOTIC DAUGHTER L-929 CELLS DURING THEIR MIGRATION
ON SUBSTRATE
Yu. P. Petrov,1,* Yu. A. Negulyaev,1,2 and N. V. Tsupkina 1
1 Institute of Cytology RAS, St.Petersburg; 2 Chair of physical and chemical biology of the Cell, St.-Petersburg state polytechnical university,
St.-Petersburg, Russia;
* e-mail: yupe3ov@mail.ru
Using time-lapse microscopy, spreading of the post-mitotic daughter cells has been studied. The work was performed on non-synchronized cells of established L-929 cell line.
The study was aimed to characterize the morphology of the cells as they move along the substrate and to determine whether the area of the migrating cells changes nonrandom.
Two new parameters have been proposed for comparison of cell morphology: the identity indicator (II) and the synchronism indicator (SI). Time-dependent changes in the area
in pairs of cells were measured to calculate these parameters. The first indicator shows the degree of coincidence between the absolute values of the area in the pair of the cells,
whereas the second indicator shows synchronism of the changes in the cell areas and does not depend on their absolute values. The lower are the indicators, the higher is the
similarity in the time-dependent changes in the areas of cell pairs studied. The indicators were shown to be approximately 1.5-fold lower for the pairs of the post-mitotic daughter
cells than those for any other pair of the cells. The results indicate a nonrandom pattern of change in the morphology of the cells during their movement along the substrate.
Key words: cell area, cellular cycle, identity indicator, synchronization indicator
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