DYNAMICS OF SPREADING OF CELLS OF L-929 LINE AFTER THE MITOSIS
Yu. P. Petrov,1,* Yu. A. Negulyaev,1,2 and N. V. Tsupkina 1
1 Institute of Cytology RAS, St.Petersburg; 2 Chair of physical and chemical biology of the cell the St.-Petersburg State Polytechnical University,
St.-Petersburg, Russia;
* e-mail: yupe3ov@mail.ru
Using time-lapse microscopy, the changes in L-929 cells shape were analyzed during a cell cycle. During this time the cells were established to pass through three spreading stages.
The highest rate of the cell spreading was observed during the first 1.5 h of mitosis. In this period, the cell area increases approximately 3-3.5 times following sigmoid dependence.
After a short plateau the augmentation of the cell area starts also as a sigmoid dependence. This period is longer (up to 6. after the beginning of cell division) with an additional 1.5-fold
augmentation of the cells size. Next, the augmentation of the cells area goes linearly up to the beginning of the following mitosis. After the mother L-929 cell division, the daughter cells
remained to be bridged together in the fission furrow site almost in 100 % cases. The structure known as an intercellular bridge is related to a late telophase. In this connected state the
L-cells are spreading and migrating up to 2.13 ± 0.06 h whereupon they are separated. Transition of the daughter cells from a round shape to the spread one occurring with the
simultaneous maintenance of the intercellular bridge during a strictly determined time allows us to consider this phenomenon as independent and not relating to mitosis. We suggest
naming this junction between the daughter cells as the «posttelophase intercellular bridge».
Key words: cell area, cell cycle, daughter cells, fission furrow, posttelophase intercellular bridge
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