CACO2 CELL CULTURE AS INTESTINAL EPITHELIUM MODEL
FOR HEXOSE TRANSPORT STUDYING
N.M. Grefner,1 L.V. Gromova,2 A.A. Gruzdkov,2 Ya.Yu. Komissarchik 1
1 Institute of Cytology RAS and 2 I.P. Pavlov Institute of Physiology RAS, St. Petersburg;
e-mail: grefner@mail.cytspb.rssi.ru
Distribution of SGLT1 and GLUT2 hexose transporters as well as that of fibrillar actin and tight junction
proteins in cultured Caco2 cells incubated in medium with different hexose concentrations has been considered.
Glucose absorption by the cells from incubation medium has been determined. Fibrillar actin was concentrated
in the microvilli and closely to tight junction. The actin distribution was not dependent on the glucose concentration.
There was no SGLT1 association with brush border actin and the transporter localization was not dependent
on the concentration of hexose. GLUT2 was localized in the basal part of Caco2 cells after low concentration
hexose load (2.5 mM). The transporter was colocalized with microvilli actin in the apical part of the cells after
high concentration hexose load (25 mM). The tight junction proteins, occludin and claudin 1, 3, 4 were not
dependent on glucose concentration. Claudin 2 was not detected in Caco2 cells. Caco2 cell culture can be used
as a model for studying of hexose transport in small intestine epithelium.
Key words: Caco2 cell, fibrillar actin, SGLT1 transporter, GLUT2 transporter, occluding, claudin 1—4,
glucose absorption
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