Tsitologiya  2012  54 (3) : 214–217
ANALYSIS OF THE CELL CYCLE DURATION OF CELLS OF PERMANENT LINE L-929

Yu.P. Petrov,1,* Yu.A. Negulyaev,1,2 N.V. Tsupkina 1

1 Institute of Cytology RAS, St. Petersburg and 2 Chair of Physical and Chemical Biology of the Cell of St. Petersburg State Polytechnical University;
* e-mail: yupe3ov@mail.ru

The direct measurement of the cell cycle duration in L-929 cells was performed using time-lapse photography. The cell cycle duration was 15.77 ± 0.08 h with a standard deviation of 1.54 ± 0.06 h. The experimental value fit to a normal distribution with a correlation coefficient 0.999. High homogeneity of this parameter and a wide range of variability of the karyotype (58—66 chromosomes) indicate that there is no correlation between these characteristics of L-929 cells. It is also shown that the difference between cell cycle durations of daughter cells tent to zero and fits by an exponent.

Key words:  cell cycle, daughter cells, biocenosis


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