RESPONSE OF CALLIPHORA VICINA LARVAL HEMOCYTES TO ABIOTIC
AND BIOTIC FOREIGN PARTICLES INJECTION
T.V. Kind
Biological Institute of St. Petersburg State University;
e-mail: tatiana.kind@mail.ru
Human erythrocytes injection into the body cavity of Calliphora vicina postfeeding larvae results to their
fast binding by thrombocytoidal fragments with agglutinates formation. There were almost none sites of lysis
and degradation of erythrocytes in agglutinates even after shape modification and strands generation. Exceptions
are zones of agglutinates with juvenile hemocytes, where destruction of erythrocytes is seen. The sequential
injection of erythrocytes and charcoal particles leads to charcoal adhesion at first to agglutinates periphery and
later to more deep stratum of cytoplasm between the erythrocytes. Under such conditions agglutinate formation
period is accompanied with morphology variations which do not influence the intensity of agglutinating reaction.
Juvenile plasmatocytes phagocytized the charcoal particles regardless of their concentration and duration of
previous contact with erythrocytes. When mixture of abiotic and biotic particles was injected into post feeding
larvae, erythrocytes and charcoal generate independent aggregations in the range of separate agglutinates. At the
same time plasmatocytes form nodules consisting of temporary cell aggregations covered with cores of non phagocytized
charcoal particles. These data testified that presumably lectin receptors responsible for foreign biotic
and abiotic particles recognition are very near but not identical for different types of hemocytes. They may be
specifical (for plasmatocytes) or integrated to different parts of cellular membrane (in thrombocytoids).
Key words: Calliphora vicina, immunity, hemolymph, hemocytes, phagocytosis, recognition
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